Quality grade

25 DNase/RNase free references of pre-filled beads tubes of 0.5, 2 and 7mL adapted to your samples to optimize your sample preparation step!

The Precellys Lysing kits range conform to lab’s standards of quality and meet DNase and RNase free purity criteria, continuously monitored by an independent laboratory.

From raw materials to finished products, all components are fully traceable and produced in a monitored environment.


 

 

Typical test protocol used in Product certification


This quality control is done by an independent laboratory.

The certification comprises of the following:
Sample consumables are washed with a sterile DNase-RNase free water called “liquid extract”. Negative controls, positive and extract controls are made to validate all steps of the different assays.

RNase detection ≤ 1x10-7 Kunitz units/μL
A pre-filled tube is washed with a sterile DNase-RNase free water. This liquid extract is transferred from the first pre-filled tube to a second. This procedure is repeated with 14 independent pre-filled tubes. The total volume of the liquid extract is transferred in a 2mL vial DNase-RNase free. 17 µL of the liquid extract are incubated overnight at +37°C with a concentration of RNA ladder. Positive controls spiked with RNase A are incubated in the same conditions. The RNA ladder is then analyzed on a 1% agarose gel with the positive controls and a negative control.
The gel is stained with Ethidium bromide. RNase contamination is indicated by degradation of RNA ladder. For samples to pass certification, the RNA bands from the “liquid extract” must correspond to the negative control, with no evidence of degradation.

 

 

DNase detection ≤ 1x10-5 Kunitz units/μL
A pre-filled tube is washed with a sterile DNase-RNase free water. This liquid extract is transferred from the first pre-filled tube to a second. This procedure is repeated with 14 independent pre-filled tubes. The total volume of the liquid extract is transferred in a 2mL vial DNase-RNase free. 27 µL of the liquid extract are incubated overnight at +37°C with a concentration of DNA ladder. Positive controls spiked with DNase are incubated in the same conditions. The DNA ladder is then analyzed on a 1.5% agarose gel with the positive controls and a negative control.
The gel is stained with Ethidium bromide. DNase contamination is indicated by degradation of DNA ladder. For samples to pass certification, the relative intensity of the DNA bands from the “liquid extract” to the batch sample must correspond to the negative control.

 

 

 

All reported result were obtained at time of lot release and are guaranteed until peel opened.

Download the test method document

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